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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
(Plasmid #164916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCfB2337
  • Backbone size w/o insert (bp) 6477
  • Total vector size (bp) 10429
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCN4(UTR)-ZEM(TF)
  • Species
    H. sapiens (human), M. musculus (mouse), S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    3952
  • Promoter PGK1 (yeast)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tccgctctaaccgaaaaggaagg
  • 3′ sequencing primer tttccctgctcgcaggtctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNTI751 pPGK1-GCN4(UTR)-ZEM(TF) was a gift from Nicholas Ingolia (Addgene plasmid # 164916 ; http://n2t.net/addgene:164916 ; RRID:Addgene_164916)

  • For your References section:

    CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes. Muller R, Meacham ZA, Ferguson L, Ingolia NT. Science. 2020 Dec 11;370(6522). pii: 370/6522/eabb9662. doi: 10.1126/science.abb9662. 10.1126/science.abb9662 PubMed 33303588
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