pCAGGS-RaichuEV-Rac1(Y40C)
(Plasmid #164903)

• Purpose: Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
• Depositing Lab: Takeshi Imai
• Publication: Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi: 10.1016/j.celrep.2021.109276.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4712
• Total vector size (bp): 7367
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RaichuEV-Rac1
• Species: H. sapiens (human)
• Insert Size (bp): 2655
• Mutation: Y40C mutation in the Rac1 domain whcih make the sensor insensitive (weak dominant negative mutant)
• Entrez Gene: RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCAG vector is derived from Addgene #14797.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry