pAH121
(Plasmid
#164901)
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PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAH121
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Backbone manufacturerHaldimann and Wanner 2001
- Total vector size (bp) 5112
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsTemperature sensitive origin of replication. Must be grown at 30C.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namephi21 Integrase
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Insert Size (bp)1143
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHaldimann and Wanner J. Bact 183, 6384-6393 (2001).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAH121 was a gift from Keith Shearwin (Addgene plasmid # 164901 ; http://n2t.net/addgene:164901 ; RRID:Addgene_164901) -
For your References section:
The loopometer: a quantitative in vivo assay for DNA-looping proteins. Hao N, Sullivan AE, Shearwin KE, Dodd IB. Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. 10.1093/nar/gkaa1284 PubMed 33511418
Map uploaded by the depositor.
