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Garnett Lab MinLibCas9 Library
(Pooled Library #164896)

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  • Purpose

    This human genome-wide CRISPR knockout library is designed with two single guide RNAs (sgRNA) per gene.

    The minimal library has been optimized through empirical assessment using comprehensive sgRNA efficiency metrics.

  • Vector Backbone

    pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W (Plasmid #153033) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 164896 MinLibCas9 Library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately.

Library Details

  • Species
    Human
  • Genes targeted
    18,761
  • gRNAs
    37,722
  • Controls
    200 non-targeting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Graphs of benchmarking data for the MinLibCas9 library. (a) Area under the receiver operating characteristic curve and (b) Average Precision scores compared to the Project Score library. (c) Schematic showing HT-29 colorectal cancer cell line transduced with the MinLibCas9 library for screening. (d) Correlation between gene-level fold-changes with MinLibCas9 and Project Score library, (e) recall of essential genes with MinLibCas9 and Project Score library. For full details, please see Gonçalves et al. Genome Biol. 2021.

Figure 1. Garnett Lab MinLibCas9 Library from Figure 2 of Gonçalves et al. Genome Biol. 2021. Image reproduced under CC-BY 4.0 International License (Link opens in a new window).

The depositing lab recommends calculating total read counts per gRNA using the count_spacers script (Link opens in a new window) from the Feng Zhang Lab.

Read distribution per gRNA can be determined using the ineq package (Link opens in a new window) in R, calculating both the Lorenzcurve and Gini coefficient.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Garnett Lab MinLibCas9 Library was a gift from Mathew Garnett (Addgene #164896)

  • For your References section:

    Minimal genome-wide human CRISPR-Cas9 library. Gonçalves E, Thomas M, Behan FM, Picco G, Pacini C, Allen F, Vinceti A, Sharma M, Jackson DA, Price S, Beaver CM, Dovey O, Parry-Smith D, Iorio F, Parts L, Yusa K, Garnett MJ Genome Biol. 2021 Jan 21;22(1):40. doi: 10.1186/s13059-021-02268-4. PubMed 33478580
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