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Addgene

Pig3 Promoter
(Plasmid #16489)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322pl-LUC
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIG3 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6100
  • Entrez Gene
    TP53I3 (a.k.a. PIG3)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pig3 promoter from -5602 to +537.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pig3 Promoter was a gift from Bert Vogelstein (Addgene plasmid # 16489 ; http://n2t.net/addgene:16489 ; RRID:Addgene_16489)

  • For your References section:

    A model for p53-induced apoptosis. Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B. Nature. 1997 Sep 18. 389(6648):300-5. 10.1038/38525 PubMed 9305847
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