TC374
(Plasmid
#164886)
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PurposePrpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepuc57
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpl28-rtTA(Q)
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TC374 was a gift from Tian Chi (Addgene plasmid # 164886 ; http://n2t.net/addgene:164886 ; RRID:Addgene_164886) -
For your References section:
A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans. Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T. iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. Epub 2018 Dec 27. 10.1016/j.isci.2018.12.023 PubMed 30634168