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Addgene

TC1316
(Plasmid #164883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc57
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dcas13d-ADARdd
  • Alt name
    CasRx (NLS-RfxCas13d-NLS) with hADAR2dd (E488Q) inserted between T558/N559
  • Alt name
    REPAIRx (Vx)
  • Species
    Other
  • Mutation
    dCas13d L560-G586 deleted
  • Promoter CMV, T7

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TC1316 was a gift from Tian Chi (Addgene plasmid # 164883 ; http://n2t.net/addgene:164883 ; RRID:Addgene_164883)

  • For your References section:

    REPAIRx, a specific yet highly efficient programmable A > I RNA base editor. Liu Y, Mao S, Huang S, Li Y, Chen Y, Di M, Huang X, Lv J, Wang X, Ge J, Shen S, Zhang X, Liu D, Huang X, Chi T. EMBO J. 2020 Nov 16;39(22):e104748. doi: 10.15252/embj.2020104748. Epub 2020 Oct 15. 10.15252/embj.2020104748 PubMed 33058207
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