TC1078-30
(Plasmid
#164878)
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Purpose30bp(mismatch17) cas13d gRNA for 1405 editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepuc57
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA for TC1405
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gRNA/shRNA sequenceunpsecified
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SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TC1078-30 was a gift from Tian Chi (Addgene plasmid # 164878 ; http://n2t.net/addgene:164878 ; RRID:Addgene_164878) -
For your References section:
REPAIRx, a specific yet highly efficient programmable A > I RNA base editor. Liu Y, Mao S, Huang S, Li Y, Chen Y, Di M, Huang X, Lv J, Wang X, Ge J, Shen S, Zhang X, Liu D, Huang X, Chi T. EMBO J. 2020 Nov 16;39(22):e104748. doi: 10.15252/embj.2020104748. Epub 2020 Oct 15. 10.15252/embj.2020104748 PubMed 33058207