TC1541A
(Plasmid
#164875)
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PurposeSplit GFP reporter expressing GFP(beta1-10) following C>U editing of the target site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepuc57
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplit GFP(beta1-10)
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SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TC1541A was a gift from Tian Chi (Addgene plasmid # 164875 ; http://n2t.net/addgene:164875 ; RRID:Addgene_164875) -
For your References section:
Programmable C-to-U RNA editing using the human APOBEC3A deaminase. Huang X, Lv J, Li Y, Mao S, Li Z, Jing Z, Sun Y, Zhang X, Shen S, Wang X, Di M, Ge J, Huang X, Zuo E, Chi T. EMBO J. 2020 Nov 16;39(22):e104741. doi: 10.15252/embj.2020104741. Epub 2020 Oct 15. 10.15252/embj.2020104741 PubMed 33058229