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Addgene

TC1681
(Plasmid #164872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc57
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAPOBEC3A fused to PspCas13b
  • Species
    Other
  • Mutation
    human apobec Y132D
  • Entrez Gene
    APOBEC3A (a.k.a. A3A, ARP3, PHRBN, bK150C2.1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TC1681 was a gift from Tian Chi (Addgene plasmid # 164872 ; http://n2t.net/addgene:164872 ; RRID:Addgene_164872)

  • For your References section:

    Programmable C-to-U RNA editing using the human APOBEC3A deaminase. Huang X, Lv J, Li Y, Mao S, Li Z, Jing Z, Sun Y, Zhang X, Shen S, Wang X, Di M, Ge J, Huang X, Zuo E, Chi T. EMBO J. 2020 Nov 16;39(22):e104741. doi: 10.15252/embj.2020104741. Epub 2020 Oct 15. 10.15252/embj.2020104741 PubMed 33058229
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