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Addgene

pID1072
(Plasmid #164869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOSIP-KT
  • Modifications to backbone
    Carries CymR gene, nad modified T5 promoter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D
  • Growth instructions
    Must be maintained in a pir+ strain
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    phiKO2 cB repressor
  • Insert Size (bp)
    609

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pID1072 is designed to be integrated into the phi21 attachment site, using the phi21 integrase helper plasmid pAH121 (Addgene # 164901).
pID1072 carries the phiKO2 cB repressor under cumate control, but can be modified to express any candidate DNA looping protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pID1072 was a gift from Keith Shearwin (Addgene plasmid # 164869 ; http://n2t.net/addgene:164869 ; RRID:Addgene_164869)

  • For your References section:

    The loopometer: a quantitative in vivo assay for DNA-looping proteins. Hao N, Sullivan AE, Shearwin KE, Dodd IB. Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. 10.1093/nar/gkaa1284 PubMed 33511418
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