pBzCas13b-gRNA-NT
(Plasmid
#164861)
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PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164861 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 4215
- Total vector size (bp) 4360
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBzCas13b-gRNA-NT
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gRNA/shRNA sequenceCTCGTATGTTGTGTGGAATTGTGAGCGGAT
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SpeciesBergeyella zoohelcum
- Promoter J23119
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer GAACCTTCGAAAAACCGCCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Spacer sequence designed for use as a negative/non-targeting control.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBzCas13b-gRNA-NT was a gift from Chase Beisel (Addgene plasmid # 164861 ; http://n2t.net/addgene:164861 ; RRID:Addgene_164861) -
For your References section:
Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems. Wandera KG, Beisel CL. Methods Mol Biol. 2022;2404:135-153. doi: 10.1007/978-1-0716-1851-6_7. 10.1007/978-1-0716-1851-6_7 PubMed 34694607