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Addgene

p281 TIR1_HA_flox STOP_mH11
(Plasmid #164856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PS100010
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 2848
  • Total vector size (bp) 10792
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OsTIR1
  • Species
    Oryza sativa (rice)
  • Insert Size (bp)
    1725
  • Promoter EF1-alpha
  • Tag / Fusion Protein
    • HA tag

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was created from p285_TIR1_HA_mH11 (ID:164855): floxed STOP cassette was subcloned from Addgene #85006 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p281 TIR1_HA_flox STOP_mH11 was a gift from Philip Jordan (Addgene plasmid # 164856 ; http://n2t.net/addgene:164856 ; RRID:Addgene_164856)

  • For your References section:

    Adaptation of the AID system for stem cell and transgenic mouse research. Pryzhkova MV, Xu MJ, Jordan PW. Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078. 10.1016/j.scr.2020.102078 PubMed 33202307
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