p285 TIR1_HA_mH11
(Plasmid #164855)

• Purpose: constitutive TIR1 expression vector for mouse H11 locus
• Depositing Lab: Philip Jordan
• Publication: Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078.

Backbone

• Vector backbone: PS100010
• Backbone manufacturer: Origene
• Backbone size w/o insert (bp): 2848
• Total vector size (bp): 9375
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsTIR1
• Species: Oryza sativa (rice)
• Insert Size (bp): 1725
• Promoter: EF1-alpha
• Tag / Fusion Protein:
  • HA tag

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • p285 TIR1_HA_mH11.gbk
• A portion of this plasmid was derived from a plasmid made by: EF1-alpha and TIR1 sequences are subcloned from pRAIDRS-P10 R. Brosh et al., Nat. Com. 2016; loxP-Puro-loxP cassette is subcloned from Addgene #22208 TV-hPITX3-Reverse; homology arms for mouse H11 locus are amplified from C57BL/6J mESCs

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry