p273 EF1a_mCherry2_hH11
(Plasmid #164852)

• Purpose: mCherry2-expression vector for human H11 locus
• Depositing Lab: Philip Jordan
• Publication: Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223-235. doi: 10.1007/s13770-020-00240-0. Epub 2020 Feb 29.

Backbone

• Vector backbone: PS100010
• Backbone manufacturer: Origene
• Backbone size w/o insert (bp): 2848
• Total vector size (bp): 8315
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry2
• Insert Size (bp): 711
• Promoter: EF1-alpha

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • p273 EF1a_mCherry2_hH11.gbk
• A portion of this plasmid was derived from a plasmid made by: mCherry2 is subcloned from Addgene #72796 pMK280 (mCherry2-NeoR); EF1-alpha is subcloned from pRAIDRS-P10 R. Brosh et al., Nat. Com. 2016; loxP-Puro-loxP cassette is subcloned from Addgene #22208 TV-hPITX3-Reverse; homology arms for human H11 are amplified from hESCs - H1 (WiCell).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry