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Addgene

p274 EF1a_EGFP_hH11
(Plasmid #164851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PS100010
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 2848
  • Total vector size (bp) 8311
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720
  • Promoter EF1-alpha

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP is subcloned from Addgene #13776 pCAG-Cre:GFP
EF1-alpha is subcloned from pRAIDRS-P10 R. Brosh et al., Nat. Com. 2016
loxP-Puro-loxP cassette is subcloned from Addgene #22208 TV-hPITX3-Reverse

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p274 EF1a_EGFP_hH11 was a gift from Philip Jordan (Addgene plasmid # 164851 ; http://n2t.net/addgene:164851 ; RRID:Addgene_164851)

  • For your References section:

    Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell and Testis Development Research. Pryzhkova MV, Jordan PW. Tissue Eng Regen Med. 2020 Apr;17(2):223-235. doi: 10.1007/s13770-020-00240-0. Epub 2020 Feb 29. 10.1007/s13770-020-00240-0 PubMed 32114677
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