pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y
(Plasmid #164845)

• Purpose: Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
• Depositing Lab: Erik Procko
• Publication: Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf1738. doi: 10.1126/sciadv.abf1738. Print 2021 Feb.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 6028
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2
• Alt name: RBD
• Species: SARS coronavirus 2
• Insert Size (bp): 696
• Mutation: Codon-optimized for human cell expression. Has N501Y mutation.
• GenBank ID: YP_009724390.1
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 8his tag (C terminal on insert)
  • HA leader sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry