pCDNA_NEPTUNE_hTLN2-FL
(Plasmid
#164842)
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PurposeExpression of NEPTUNE tagged FL human talin2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN2
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SpeciesH. sapiens (human)
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Entrez GeneTLN2 (a.k.a. ILWEQ)
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Tag
/ Fusion Protein
- His10-NEPTUNE (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA_NEPTUNE_hTLN2-FL was a gift from Tina Izard (Addgene plasmid # 164842 ; http://n2t.net/addgene:164842 ; RRID:Addgene_164842) -
For your References section:
A distinct talin2 structure directs isoform specificity in cell adhesion. Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard T. J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 10.1074/jbc.RA119.010789. Epub 2020 Jun 30. 10.1074/jbc.RA119.010789 PubMed 32605925