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Addgene

pET28_hTLN2-head-LBD
(Plasmid #164837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLN2
  • Species
    H. sapiens (human)
  • Mutation
    Lipid binding deficient mutations
  • Entrez Gene
    TLN2 (a.k.a. ILWEQ)
  • Tag / Fusion Protein
    • His8 (N terminal on backbone)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_hTLN2-head-LBD was a gift from Tina Izard (Addgene plasmid # 164837 ; http://n2t.net/addgene:164837 ; RRID:Addgene_164837)

  • For your References section:

    A distinct talin2 structure directs isoform specificity in cell adhesion. Rangarajan ES, Primi MC, Colgan LA, Chinthalapudi K, Yasuda R, Izard T. J Biol Chem. 2020 Sep 11;295(37):12885-12899. doi: 10.1074/jbc.RA119.010789. Epub 2020 Jun 30. 10.1074/jbc.RA119.010789 PubMed 32605925
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