pTY1-LacI
(Plasmid
#164831)
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PurposeLacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAN1818
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MG1655dLac
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLacI
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Alt namelac repressor
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SpeciesE. coli
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Insert Size (bp)1083
- Promoter Ptac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CAATTAATCATCGGCTCGTATAATGTGTGGAATT
- 3′ sequencing primer TGCAGCGAGTCAGTGAGCGAGGAAGCACCTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTY1-LacI was a gift from Drew Tack (Addgene plasmid # 164831 ; http://n2t.net/addgene:164831 ; RRID:Addgene_164831) -
For your References section:
The genotype-phenotype landscape of an allosteric protein. Tack DS, Tonner PD, Pressman A, Olson ND, Levy SF, Romantseva EF, Alperovich N, Vasilyeva O, Ross D. Mol Syst Biol. 2021 Mar;17(3):e10179. doi: 10.15252/msb.202010179. 10.15252/msb.202010179 PubMed 33784029