pVER-LacI
(Plasmid #164830)

• Purpose: LacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.
• Depositing Lab: Drew Tack
• Publication: Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. doi: 10.15252/msb.202010179.

Backbone

• Vector backbone: pAN1818
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MG1655
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LacI
• Alt name: lac repressor
• Species: E. coli
• Insert Size (bp): 1083
• Promoter: Ptac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CTCTCGGCATGGACGAGCTGT
• 3′ sequencing primer: TGCAGCGAGTCAGTGAGCGAGGAAGCACCTC

Resource Information

• Supplemental Documents:
  • pVER-lacI.gb
  • pVER-lacI.gb
• A portion of this plasmid was derived from a plasmid made by: derivative of pAN1818 plasmid
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from the pAN1818 plasmid from the Chris Voigt lab.