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Addgene

pET-MAT2A
(Plasmid #164820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) bacterial strain for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methionine Adenosyltransferase 2A
  • Alt name
    MAT2A
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAT2A (a.k.a. MATA2, MATII, SAMS2)
  • Tag / Fusion Protein
    • 6x His Tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-MAT2A was a gift from Vern Schramm (Addgene plasmid # 164820 ; http://n2t.net/addgene:164820 ; RRID:Addgene_164820)

  • For your References section:

    The Transition-State Structure for Human MAT2A from Isotope Effects. Firestone RS, Schramm VL. J Am Chem Soc. 2017 Oct 4;139(39):13754-13760. doi: 10.1021/jacs.7b05803. Epub 2017 Sep 20. 10.1021/jacs.7b05803 PubMed 28880543
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