pU6-mir144~451 EF1Alpha-puro-T2A-BFP
(Plasmid #164793)

• Purpose: Express miR-144~451 under the U6 promoter in mammalian cells
• Depositing Lab: David Bartel
• Publication: Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 10.1016/j.molcel.2020.01.026.

Backbone

• Vector backbone: pU6-sgRNA EF1Alpha-puro-T2A-BFP
• Backbone manufacturer: Jonathan Weissman (Addgene plasmid # 60955)
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: miR-144~451
• Species: H. sapiens (human)
• Entrez Gene: MIR451A (a.k.a. MIR451, MIRN451, hsa-mir-451, hsa-mir-451a, mir-451a)
• Entrez Gene: MIR144 (a.k.a. MIRN144, mir-144)
• Promoter: U6

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: U6_seq

Gene/Insert 2

• Gene/Insert name: puro-T2A-BFP
• Promoter: EF1Alpha

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: EF1Alpha sequencing primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry