pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA
(Plasmid
#164787)
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PurposepLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti7.3DEST
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Backbone manufacturerInvitrogen
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Modifications to backboneModified the backbone by removing the emGFP expression cassette
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGluN2B
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 164787 ; http://n2t.net/addgene:164787 ; RRID:Addgene_164787) -
For your References section:
The production of viral vectors designed to express large and difficult to express transgenes within neurons. Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE. Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. 10.1186/s13041-015-0100-7 PubMed 25887710