GLuc-PM-Venus
(Plasmid #164785)

• Purpose: Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
• Depositing Lab: Laszlo Hunyady
• Publication: Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3959
• Total vector size (bp): 5507
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus
• Alt name: GLuc
• Alt name: PDGFRB
• Alt name: YFP
• Species: H. sapiens (human); Gaussia princeps
• Insert Size (bp): 1548
• Entrez Gene: PDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GLuc (N terminal on insert)
  • mVenus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII, XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer

Resource Information

• Supplemental Documents:
  • pEYFP-N1 GLuc-PM-Venus.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry