NanoLuc-PM
(Plasmid #164784)

• Purpose: Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
• Depositing Lab: Laszlo Hunyady
• Publication: Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.1016/j.jbc.2021.100366.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5380
• Total vector size (bp): 6196
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta
• Alt name: NanoLuc
• Alt name: PDGFRB
• Species: H. sapiens (human); Oplophorus gracilirostris
• Insert Size (bp): 816
• Entrez Gene: PDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)
• Promoter: CMV
• Tag / Fusion Protein:
  • NanoLuc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII, AgeI (not destroyed)
• 3′ cloning site: NotI, XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: BGH_rev_primer

Resource Information

• Supplemental Documents:
  • pcDNA3.1 NanoLuc-PM.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry