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Addgene

pcDNA3-CMV-Grid2(I677C)
(Plasmid #164782)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164782 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grid2 I677C
  • Alt name
    LiGluD2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3027
  • Mutation
    I677C
  • Entrez Gene
    Grid2 (a.k.a. B230104L07Rik, GluD2, GluRdelta2, Lc, Lc<J>, MMS10-AC, Ms10ac, cpr, ho, nmf408, tpr)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-CMV-Grid2(I677C) was a gift from Alexandre Mourot (Addgene plasmid # 164782 ; http://n2t.net/addgene:164782 ; RRID:Addgene_164782)
  • For your References section:

    Probing the ionotropic activity of glutamate GluD2 receptor in HEK cells with genetically-engineered photopharmacology. Lemoine D, Mondoloni S, Tange J, Lambolez B, Faure P, Taly A, Tricoire L, Mourot A. Elife. 2020 Oct 28;9. pii: 59026. doi: 10.7554/eLife.59026. 10.7554/eLife.59026 PubMed 33112237