pcDNA3-CMV-Grid2(I677C)
(Plasmid
#164782)
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Purposecysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGrid2 I677C
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Alt nameLiGluD2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3027
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MutationI677C
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Entrez GeneGrid2 (a.k.a. B230104L07Rik, GluD2, GluRdelta2, Lc, Lc<J>, MMS10-AC, Ms10ac, cpr, ho, nmf408, tpr)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-CMV-Grid2(I677C) was a gift from Alexandre Mourot (Addgene plasmid # 164782 ; http://n2t.net/addgene:164782 ; RRID:Addgene_164782) -
For your References section:
Probing the ionotropic activity of glutamate GluD2 receptor in HEK cells with genetically-engineered photopharmacology. Lemoine D, Mondoloni S, Tange J, Lambolez B, Faure P, Taly A, Tricoire L, Mourot A. Elife. 2020 Oct 28;9. pii: 59026. doi: 10.7554/eLife.59026. 10.7554/eLife.59026 PubMed 33112237