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pIRES2-CMV-nAChRb2(E61C)-IRES-GFP
(Plasmid #164779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone size w/o insert (bp) 3728
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nAChR b2 E61C - IRES- GFP
  • Alt name
    LinAChR b2 -IRES- GFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3116
  • Mutation
    E61C
  • Entrez Gene
    Chrnb2 (a.k.a. Acrb-2, Acrb2, C030030P04Rik, [b]2-nAchR)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-CMV-nAChRb2(E61C)-IRES-GFP was a gift from Alexandre Mourot (Addgene plasmid # 164779 ; http://n2t.net/addgene:164779 ; RRID:Addgene_164779)

  • For your References section:

    Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. Durand-de Cuttoli R, Mondoloni S, Marti F, Lemoine D, Nguyen C, Naude J, d'Izarny-Gargas T, Pons S, Maskos U, Trauner D, Kramer RH, Faure P, Mourot A. Elife. 2018 Sep 4;7. pii: 37487. doi: 10.7554/eLife.37487. 10.7554/eLife.37487 PubMed 30176987
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