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Addgene

pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA
(Plasmid #164776)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164776 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti7.3DEST
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Modified the backbone by removing the emGFP expression cassette and adding multiple cloning site (MCS) via ClaI and MluI sites.
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GluN2A
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA was a gift from Jonathan Ploski (Addgene plasmid # 164776 ; http://n2t.net/addgene:164776 ; RRID:Addgene_164776)
  • For your References section:

    The production of viral vectors designed to express large and difficult to express transgenes within neurons. Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE. Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. 10.1186/s13041-015-0100-7 PubMed 25887710