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Addgene

pSG5 PMS2-134
(Plasmid #16476)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMS2 1-134
  • Alt name
    PMS2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Mutation
    Codons 1-134
  • Entrez Gene
    PMS2 (a.k.a. HNPCC4, LYNCH4, MLH4, MMRCS4, PMS-2, PMS2CL, PMSL2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5 PMS2-134 was a gift from Bert Vogelstein (Addgene plasmid # 16476 ; http://n2t.net/addgene:16476 ; RRID:Addgene_16476)

  • For your References section:

    A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. Nicolaides NC, Littman SJ, Modrich P, Kinzler KW, Vogelstein B. Mol Cell Biol. 1998 Mar . 18(3):1635-41. PubMed 9488480
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