LjUBQ:MtLEGIN13:T35s
(Plasmid
#164750)
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PurposeOverexpression of Peptide coding gene (Binary vector)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEC50505
- Backbone size w/o insert (bp) 10475
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418) ; N/A
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMedtr3g436100.1
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Alt nameLEGIN13
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Alt nameLOC25490436
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SpeciesMedicago truncatula
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Insert Size (bp)979
- Promoter Lotus japonicus Ubiquitin
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Tag
/ Fusion Protein
- Terminator 35S (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned by Peter/Shulan, additional dsRED selection
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LjUBQ:MtLEGIN13:T35s was a gift from Wolf Scheible (Addgene plasmid # 164750 ; http://n2t.net/addgene:164750 ; RRID:Addgene_164750)