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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4076
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePMS2
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SpeciesH. sapiens (human)
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Insert Size (bp)2800
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Entrez GenePMS2 (a.k.a. HNPCC4, LYNCH4, MLH4, MMRCS4, PMS-2, PMS2CL, PMSL2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5 PMS2-wt was a gift from Bert Vogelstein (Addgene plasmid # 16475 ; http://n2t.net/addgene:16475 ; RRID:Addgene_16475) -
For your References section:
A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype. Nicolaides NC, Littman SJ, Modrich P, Kinzler KW, Vogelstein B. Mol Cell Biol. 1998 Mar . 18(3):1635-41. PubMed 9488480