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Addgene

pYPQ265F2
(Plasmid #164722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ255
  • Total vector size (bp) 8367
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible A3A/Y130F-nCas9-NG-CBE_V01 entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A(Y130F)-zCas9(D10A)NG-UGI

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ265F2 was a gift from Yiping Qi (Addgene plasmid # 164722 ; http://n2t.net/addgene:164722 ; RRID:Addgene_164722)

  • For your References section:

    Improved plant cytosine base editors with high editing activity, purity, and specificity. Ren Q, Sretenovic S, Liu G, Zhong Z, Wang J, Huang L, Tang X, Guo Y, Liu L, Wu Y, Zhou J, Zhao Y, Yang H, He Y, Liu S, Yin D, Mayorga R, Zheng X, Zhang T, Qi Y, Zhang Y. Plant Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635. 10.1111/pbi.13635 PubMed 34042262
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