pYPQ269A2
(Plasmid
#164714)
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PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ265
- Total vector size (bp) 9342
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Vector typePlant Expression, CRISPR ; Gateway compatible rAPOBEC1-CBE_V04 entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ269A2 was a gift from Yiping Qi (Addgene plasmid # 164714 ; http://n2t.net/addgene:164714 ; RRID:Addgene_164714) -
For your References section:
Improved plant cytosine base editors with high editing activity, purity, and specificity. Ren Q, Sretenovic S, Liu G, Zhong Z, Wang J, Huang L, Tang X, Guo Y, Liu L, Wu Y, Zhou J, Zhao Y, Yang H, He Y, Liu S, Yin D, Mayorga R, Zheng X, Zhang T, Qi Y, Zhang Y. Plant Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635. 10.1111/pbi.13635 PubMed 34042262