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Addgene

pCL10
(Plasmid #164710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS406
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET3pr - yEVenus - CLN2 PEST - ADH1t
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site PacI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL10 was a gift from Fred Cross (Addgene plasmid # 164710 ; http://n2t.net/addgene:164710 ; RRID:Addgene_164710)

  • For your References section:

    Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor. Gligorovski V, Sadeghi A, Rahi SJ. Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8. 10.1038/s41467-023-38959-8 PubMed 37369667
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