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pVG48
(Plasmid #164706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EZL105
  • Backbone manufacturer
    Zhou et al, Nature, 2018 PMID: 29562237
  • Modifications to backbone
    The light-inducible transcription factor EL222 has been replaced with rTA.
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGK1pr - rtTA - CYC1t
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    rtTA was cloned from pCH043 which was a gift from Attila Becskei

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVG48 was a gift from Sahand Jamal Rahi (Addgene plasmid # 164706 ; http://n2t.net/addgene:164706 ; RRID:Addgene_164706)

  • For your References section:

    Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor. Gligorovski V, Sadeghi A, Rahi SJ. Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8. 10.1038/s41467-023-38959-8 PubMed 37369667
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