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Addgene

PRMT8(E185Q)-FLAG
(Plasmid #164700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT8
  • Alt name
    HRMT1L3
  • Alt name
    HRMT1L4
  • Species
    H. sapiens (human)
  • Mutation
    E185Q
  • Entrez Gene
    PRMT8 (a.k.a. HRMT1L3, HRMT1L4)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV forward: 5' CGCAAATGGGCGGTAGGCGTG 3'
  • 3′ sequencing primer BGH: 5' TAGAAGGCACAGTCGAGG 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRMT8(E185Q)-FLAG was a gift from Cheryl Arrowsmith (Addgene plasmid # 164700 ; http://n2t.net/addgene:164700 ; RRID:Addgene_164700)
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