pCK-Drosha(UniProt)-FLAG-mEGFP
(Plasmid
#164691)
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PurposeExpress Drosha-FLAG-mEGFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Backbone manufacturerN/A
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosha
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SpeciesH. sapiens (human)
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Entrez GeneDROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
- Promoter CMV
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Tags
/ Fusion Proteins
- FLAG (C terminal on insert)
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Derived from pCK-Drosha-FLAG published in Han, J., Lee, Y., Yeom, K.H., Kim, Y.K., Jin, H., and Kim, V.N. (2004). The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev 18, 3016-3027.
Substitutions made: P30S, V135A, G200S, L321S
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCK-Drosha(UniProt)-FLAG-mEGFP was a gift from David Bartel (Addgene plasmid # 164691 ; http://n2t.net/addgene:164691 ; RRID:Addgene_164691) -
For your References section:
MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein. Fang W, Bartel DP. Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 10.1016/j.molcel.2020.01.026. 10.1016/j.molcel.2020.01.026 PubMed 32302541