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Addgene

pCZ45ai
(Plasmid #16469)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCZ
  • Backbone size w/o insert (bp) 9174
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53 binding site
  • Alt name
    p53
  • Species
    H. sapiens (human)
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information. pCZ-PG-betaGal, PG: a p53 binding site consisting of 16 copies of a recognition motif.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZ45ai was a gift from Bert Vogelstein (Addgene plasmid # 16469 ; http://n2t.net/addgene:16469 ; RRID:Addgene_16469)

  • For your References section:

    PAK1, a gene that can regulate p53 activity in yeast. Thiagalingam S, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1995 Jun 20. 92(13):6062-6. 10.1073/pnas.92.13.6062 PubMed 7597081
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