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Addgene

pTA231-p.Syn-PAmCherry1Hfx
(Plasmid #164661)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164661 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTA231-p.Syn
  • Backbone manufacturer
    Berkemer et al., 2020, Identification of RNA 3´ ends and termination sites in Haloferax volcanii
  • Backbone size w/o insert (bp) 7765
  • Total vector size (bp) 8476
  • Vector type
    archaeal expression
  • Selectable markers
    marker for H. volcanii: trpA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmCherry1Hfx
  • Alt name
    H. volcanii codon-optimized PAmCherry1
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Mutation
    codon-optimization for Haloferax volcanii
  • Promoter p.Syn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PAmCherry1Hfx ordered from GeneArt for synthesis

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA231-p.Syn-PAmCherry1Hfx was a gift from Anita Marchfelder (Addgene plasmid # 164661 ; http://n2t.net/addgene:164661 ; RRID:Addgene_164661)
  • For your References section:

    Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii. Turkowyd B, Schreiber S, Wortz J, Segal ES, Mevarech M, Duggin IG, Marchfelder A, Endesfelder U. Front Microbiol. 2020 Nov 20;11:583010. doi: 10.3389/fmicb.2020.583010. eCollection 2020. 10.3389/fmicb.2020.583010 PubMed 33329447