pTA231-p.Syn-PAmCherry1Hfx
(Plasmid #164661)

• Purpose: expression of H. volcanii codon-optimized PAmCherry1
• Depositing Lab: Anita Marchfelder
• Publication: Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010. doi: 10.3389/fmicb.2020.583010. eCollection 2020.

Backbone

• Vector backbone: pTA231-p.Syn
• Backbone manufacturer: Berkemer et al., 2020, Identification of RNA 3´ ends and termination sites in Haloferax volcanii
• Backbone size w/o insert (bp): 7765
• Total vector size (bp): 8476
• Vector type: archaeal expression
• Selectable markers: marker for H. volcanii: trpA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAmCherry1Hfx
• Alt name: H. volcanii codon-optimized PAmCherry1
• Species: Synthetic
• Insert Size (bp): 711
• Mutation: codon-optimization for Haloferax volcanii
• Promoter: p.Syn

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PAmCherry1Hfx ordered from GeneArt for synthesis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry