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Addgene

pCS2-npas4l
(Plasmid #164654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4063
  • Total vector size (bp) 6007
  • Vector type
    mRNA synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    npas4l
  • Alt name
    clo
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1944
  • Mutation
    Please see depositor comments
  • Entrez Gene
    npas4l (a.k.a. c, clo, cloc)
  • Promoter SP6

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    cloned by Michele Marass Genome-wide strategies reveal target genes of Npas4l associated with vascular development in zebrafish Michele Marass, Arica Beisaw, Claudia Gerri, Francesca Luzzani, Nana Fukuda, Stefan Günther, Carsten Kuenne, Sven Reischauer, Didier Y. R. Stainier Development 2019 146: dev173427 doi: 10.1242/dev.173427 Published 3 June 2019
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS result had deletion of amino acids 349-352 (TSSS). Depositor confirmed that this deletion does not affect the plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-npas4l was a gift from Didier Stainier (Addgene plasmid # 164654 ; http://n2t.net/addgene:164654 ; RRID:Addgene_164654)

  • For your References section:

    Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2. Mattonet K, Riemslagh FW, Guenther S, Prummel KD, Kesavan G, Hans S, Ebersberger I, Brand M, Burger A, Reischauer S, Mosimann C, Stainier DYR. Sci Adv. 2022 Sep 2;8(35):eabn2082. doi: 10.1126/sciadv.abn2082. Epub 2022 Aug 31. 10.1126/sciadv.abn2082 PubMed 36044573
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