sgRNA(MS2)_zeo insert c-MYC
(Plasmid #164636)

• Purpose: sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
• Depositing Lab: Günter Schneider
• Publication: sgRNA MS2 (zeo_backbone) target c-MYC (unpublished)

Backbone

• Vector backbone: sgRNA(MS2)_zeo #61427
• Modifications to backbone: insert was cloned into BsmBI site
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: target sequence for c-Myc activation
• Alt name: Myc proto-oncogene protein
• Species: H. sapiens (human)
• Insert Size (bp): 25
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Promoter: U6 and EF1A

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CACCGTTCCCCCACGCCCTCTGCTT
• 3′ sequencing primer: AAACAAGCAGAGGGCGTGGGGGAAC

Resource Information

• Supplemental Documents:
  • Protocol for cloning target sequence into sgRNA backbone

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry