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Addgene

3xflag-APEX2-LRRK2
(Plasmid #164622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV-3xFLAG-APEX2-pDEST
  • Backbone size w/o insert (bp) 7180
  • Total vector size (bp) 13136
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRRK2
  • Alt name
    Leucine-rich repeat serine/threonine-protein kinase 2
  • Alt name
    PARK8
  • Alt name
    Dardarin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7584
  • GenBank ID
    NM_198578.4 NM_198578.4
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • APEX2 (N terminal on insert)
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAG
  • 3′ sequencing primer TTACTCAACAGATGTTCGTCTCATTTTTTCAGCTAATTCTTTTCTCACTTCAATGTGTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xflag-APEX2-LRRK2 was a gift from Mark Cookson (Addgene plasmid # 164622 ; http://n2t.net/addgene:164622 ; RRID:Addgene_164622)

  • For your References section:

    LRRK2 mediates tubulation and vesicle sorting from lysosomes. Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, Cookson MR. Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 10.1126/sciadv.abb2454. Print 2020 Nov. 10.1126/sciadv.abb2454 PubMed 33177079
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