-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16462 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepJFCAT1
-
Backbone manufacturerATCC
- Backbone size w/o insert (bp) 5600
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namep21 promoter
-
Alt nameWAF1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2280
-
GenBank IDU03106
-
Entrez GeneCDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Human p21 promoter deletion-CAT reporter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p21 promoter A was a gift from Bert Vogelstein (Addgene plasmid # 16462 ; http://n2t.net/addgene:16462 ; RRID:Addgene_16462) -
For your References section:
Topological control of p21WAF1/CIP1 expression in normal and neoplastic tissues. el-Deiry WS, Tokino T, Waldman T, Oliner JD, Velculescu VE, Burrell M, Hill DE, Healy E, Rees JL, Hamilton SR. Cancer Res. 1995 Jul 1. 55(13):2910-9. PubMed 7796420