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pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
(Plasmid #164615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF4
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    Renilla reniformis
  • Promoter TetCMV
  • Tags / Fusion Proteins
    • SunTag (N terminal on insert)
    • 24xMS2v5 (3'UTR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site RsrII (not destroyed)
  • 5′ sequencing primer Ggctcgtactctataggc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA was a gift from Jeffrey Chao (Addgene plasmid # 164615 ; http://n2t.net/addgene:164615 ; RRID:Addgene_164615)

  • For your References section:

    Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules. Mateju D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao JA. Cell. 2020 Dec 23;183(7):1801-1812.e13. doi: 10.1016/j.cell.2020.11.010. Epub 2020 Dec 11. 10.1016/j.cell.2020.11.010 PubMed 33308477
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