pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
(Plasmid
#164614)
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PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE-UbiC
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namestdMCP-stdHalo-RH1
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SpeciesSynthetic
- Promoter UbiC
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- HA tag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GTGTGGCACAGCTAGTTCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1 was a gift from Jeffrey Chao (Addgene plasmid # 164614 ; http://n2t.net/addgene:164614 ; RRID:Addgene_164614) -
For your References section:
Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules. Mateju D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao JA. Cell. 2020 Dec 23;183(7):1801-1812.e13. doi: 10.1016/j.cell.2020.11.010. Epub 2020 Dec 11. 10.1016/j.cell.2020.11.010 PubMed 33308477