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Addgene

pCMV-sypHTomato
(Plasmid #164613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A reporter of vesicular turnover sypHTomato
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1770
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-sypHTomato was a gift from Yulong Li & Richard Tsien (Addgene plasmid # 164613 ; http://n2t.net/addgene:164613 ; RRID:Addgene_164613)

  • For your References section:

    pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity. Li Y, Tsien RW. Nat Neurosci. 2012 May 27;15(7):1047-53. doi: 10.1038/nn.3126. 10.1038/nn.3126 PubMed 22634730
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