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Addgene

pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX
(Plasmid #164612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Endocannabinoid non-responsive sensor GRAB_eCBmut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2217
  • Mutation
    S383T, F177A
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX was a gift from Yulong Li (Addgene plasmid # 164612 ; http://n2t.net/addgene:164612 ; RRID:Addgene_164612)

  • For your References section:

    A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo. Dong A, He K, Dudok B, Farrell JS, Guan W, Liput DJ, Puhl HL, Cai R, Wang H, Duan J, Albarran E, Ding J, Lovinger DM, Li B, Soltesz I, Li Y. Nat Biotechnol. 2021 Nov 11. pii: 10.1038/s41587-021-01074-4. doi: 10.1038/s41587-021-01074-4. 10.1038/s41587-021-01074-4 PubMed 34764491
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