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pCMV DCC mut
(Plasmid #16460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Neo-XhoI
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • Mutation
    5' end of DCC
  • Entrez Gene
    DCC (a.k.a. CRC18, CRCR1, HGPPS2, IGDCC1, MRMV1, NTN1R1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has a frame-shift at nt 189, so that it encodes only the 5' end of DCC (3.5 kb) and is missing the entire intracytoplasmic domain. The actual insert between cloning sites is still 4.4kb (see review digest).

Additional reference: Cho KR et al, The DCC Gene: Structural analysis and mutations in colorectal carcinomas. Genomics 19: 525-531, 1994.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV DCC mut was a gift from Bert Vogelstein (Addgene plasmid # 16460 ; http://n2t.net/addgene:16460 ; RRID:Addgene_16460)

  • For your References section:

    Identification of a chromosome 18q gene that is altered in colorectal cancers. Fearon ER, Cho KR, Nigro JM, Kern SE, Simons JW, Ruppert JM, Hamilton SR, Preisinger AC, Thomas G, Kinzler KW. Science. 1990 Jan 5. 247(4938):49-56. 10.1126/science.2294591 PubMed 2294591
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