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Addgene

pLVX-IRES-Hygro_GCaMP6s
(Plasmid #164591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-IRES-Hygro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8513
  • Total vector size (bp) 9866
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6s
  • Species
    R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
  • Insert Size (bp)
    1443
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer IRES Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-IRES-Hygro_GCaMP6s was a gift from Joseph Hyser (Addgene plasmid # 164591 ; http://n2t.net/addgene:164591 ; RRID:Addgene_164591)

  • For your References section:

    Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum. Chang-Graham AL, Perry JL, Strtak AC, Ramachandran NK, Criglar JM, Philip AA, Patton JT, Estes MK, Hyser JM. Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8. 10.1038/s41598-019-46856-8 PubMed 31346185
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